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  • Peak splitting tends to occur in all peaks with column head collapse.
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    Peak tailing.

  • HPLC Troubleshooting Guide.
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    4 % FA and acetonitrile containing 0.

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    Note that injection volume is limited by column size; general guidelines for acceptable ranges are as follows: Column ID.

  • Below is a typical method with B = eluting solvent and rotor positions 1-2 to column.
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    Finding the root cause of the problem within the complex environment of an HPLC analysis is much more challenging.

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